Local Mechanical Stimulation of Mardin-Darby Canine Kidney Cell Sheets on Temperature-Responsive Hydrogel

Kurte

Collective motion of cell sheets plays a role not only in development and repair, but also in devastating diseases such as cancer. However, unlike single-cell motility, collective motion of cell sheets involves complex cell-cell communication during migration; therefore, its mechanism is largely unknown. To elucidate propagation of signaling transduced by cell-cell interaction, we designed a hydrogel substrate that can cause local mechanical stretching of cell sheets. Poly (N-isopropyl acrylamide) (PNIPAAm) hydrogel is a temperature-responsive polymer gel whose volume changes isotropically in response to temperature changes below 37 °C. We designed a combined hydrogel substrate consisting of collagen-immobilized PNIPAAm as the local stimulation side and polyacrylamide (PAAm) as the non-stimulation side to assess propagation of mechanical transduction. Mardin-Darby canine kidney (MDCK) cells adhered to the collagen-immobilized PNIPAAm gel increased it area and were flattened as the gel swelled with temperature decrease. E-cadherin in these cells became undetectable in some domains, and actin stress fibers were more clearly observed at the cell base. In contrast, E-cadherin in cells adhered to the collagen-immobilized PAAm side was equally stained as that in cells adhered to the collagen-immobilized PAAm side even after temperature decrease. ERK1/2 MAPK activation of cells on the non-stimulated substrate occurred after partial stretching of the cell sheet suggesting the propagation of signaling. These results indicate that a change in the balance of mechanical tension induced by partial stretching of cell sheets leads to activation and propagation of the cell signaling.

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Jêgirtin

@article{localmechanicalstimulationofma2012,
  title = {{Local Mechanical Stimulation of Mardin-Darby Canine Kidney Cell Sheets on Temperature-Responsive Hydrogel}},
  author = {Harada, Ichiro and Yanagisawa, Shunpei and Iwasaki, Katsuhiko and Cho, Chong‐Su and Akaike, Toshihiro},
  year = {2012},
  journal = {{International Journal of Molecular Sciences}},
  volume = {13},
  number = {1},
  pages = {1095-1108},
  publisher = {{Multidisciplinary Digital Publishing Institute}},
  doi = {10.3390/ijms13011095}
}

TY  - JOUR
AU  - Harada, Ichiro
AU  - Yanagisawa, Shunpei
AU  - Iwasaki, Katsuhiko
AU  - Cho, Chong‐Su
AU  - Akaike, Toshihiro
TI  - Local Mechanical Stimulation of Mardin-Darby Canine Kidney Cell Sheets on Temperature-Responsive Hydrogel
PY  - 2012
JO  - International Journal of Molecular Sciences
VL  - 13
IS  - 1
SP  - 1095
EP  - 1108
PB  - Multidisciplinary Digital Publishing Institute
DO  - 10.3390/ijms13011095
ER  - 

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